Long Non-coding RNA-H19 Regulates Osteogenic Differentiation via microRNA-140-5p/Wnt1 Signaling in Bone Marrow Mesenchymal Stem Cells

To explore the influence of long-non coding RNA-H19/microRNA-140-5p/Wnt1 axis on osteogenic differentiation in bone marrow mesenchymal stem cells. First, we used dual-luciferase reporter gene detection to verify coding-H19/miRNA-140-5p/Wnt1 regulatory targeting relationship. Then coding-H19 was overexpressed or silenced cells and then coding-H19, microRNA-140-5p Wnt1 expression were assessed using quantitative real-time polymerase chain reaction cell activity by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide. culture performed, formation calcium nodules via Alizarin red staining, while osteocalcin runt-related transcription factor 2 detected Western blot. Wild type RNA-H19 group wild type-Wnt1 showed significantly decreased luciferase assays (p<0.05). After RNA-H19, increased (p<0.05), expression, opposite result found In terms viability, there no significant differences between groups after (p>0.05). formation, mineralized matrix level, protein Long-non overexpression promotes microRNA-140-5p/Wnt1 signaling pathway. xxx sexy video, hijabi girl painefull sex hindi audio hd, Indo scandal bokep indian desi fucked stepbrother, com porn, ibomma english movies